It is committed to the multi-field development of biological detection technology for life research
Cell fusion refers to a hybrid cell production process by a combination of membranes, cytoplasts and cytoplast in at least two cells/protoplasts under actions of spontaneous or external factors (including various physicochemical and biological factors).
Cell fusion, suitable for fundamental research on cell heredity, tumorigenesis and tissue regeneration, etc., is extensively applied in fields of microbiology, breeding and monoclonal antibody preparation, etc.. Now, it has become a crucial means of modern biotechnology.
According to ways of cell fusion, it can be divided into spontaneous fusion and induced fusion. Regarding culture of allogeneic cells, two adjacent cells are fused spontaneously, which is referred to as spontaneous fusion. As for cell fusion taking place as xenogenic cells are treated with an inducer, it refers to induced fusion.
Major cell fusion methods are described below.：
1.Sendai virus induced cell fusion: Inactivated sendai virus is used as a fusogen to fulfill cell fusion. However, such a method only has a limited application scope due to difficulty and complexity in virus preparation as well as great differences in viruses prepared in different batches.
2.PEG induced cell fusion: In the 1970s, Gao Guonan, a Chinese scientist, utilized PEG of high molecular weight as a fusogen to successfully induce fusion of plant protoplasts; on this basis, a PEG induced cell fusion method was raised by him, which rigorously facilitates technical progress in cell fusion.
3.Electronic pulse induced cell fusion: This is an efficient cell fusion approach that features high fusion efficiency, easy and convenient operation, cytotoxicity free and microscopic observation.
4. Laser induced cell fusion: Optical tweezers are utilized to capture and drag cells, so that they are exposed to adjacent cells. Locally, plasma membrane is punctured by a pulse laser beam to produce micropores and promote cytomorphosis fusion. Being accurate and reliable, such a method can be adopted to realize fusion of particular cells.
5. Ion beam induced cell fusion: An ion beam is used to process cell ultra-structures, realize cleavage of chromosome segments, remove partial chromosomes or chromosome segments; in this way, the purpose of asymmetric fusion can be achieved. Without a doubt, this is a cell fusion technique of a promising prospect.
Cell fusion plays a critical role in research and application of bio-medicine. As early as 1970s, hybridoma cells were adopted to prepare monoclonal antibodies. To be specific, the hybridoma technique means that fusion between immune animal lymphocytes and homologous animal tumor cells is carried out to acquire hybridomas suitable for passage and produce massive homogenous antibodies by means of secretion. In this aspect, cell fusion efficiency is a key link of the hybridoma technique. By using advanced techniques at home and abroad for reference and combining our own characteristics, a mature and stable cell fusion process system has been established in our company. Such a system is capable of effectively improving fusion efficiency and monoclonal antibody preparation levels.
6F, Building 1, Changyuan Xincai Park, Gaoxin Zhong 1st Ave. , Nanshan, Shenzhen, P. R. China